Abstract
Background: Cervical cancer is one of the most common causes of cancer death in women worldwide; Radiotherapy has a major role in cervical cancer treatment. Anti-cancer effects of other species of Artemisia have been shown in some human cancer cells. 
Objective: To determine the cytotoxic and radiosensitizing effects of Artemisia kopetdaghensis extract on cervical cancer HeLa cells. 
Methods: Different concentrations of Artemisia kopetdaghensis extract (ART) (25-250µg/ml) were examined on HeLa cell line. Cell cytotoxicity of the extract and combination of extract plus 2Gy radiation was evaluated after 24, 48 and 72 hours via MTT assay. ART induced apoptosis was estimated with flow cytometry after 24h. One-way analysis of variance (ANOVA) and then Bonferroni post hoc test were applied for statistical analysis. Prism (v.6) was used for all statistical analyses. 
Results: Artemisia kopetdaghensis decreased HeLa cells viability according to its concentration and timing of treatment. Comparing with the control group, a sub-G1 peak in the flow cytometry histogram of A. kopetdaghensis treated cells was shown, demonstrating that apoptosis was involved in A. kopetdaghensis cytotoxicity. Also, A. kopetdaghensis extract combined with irradiation, induced an additive cytotoxic effect on HeLa cells. 
Conclusion: Artemisia kopetdaghensis extract might be considered as a radiosensitizer in cervical cancer treatment potentially, and can be a good candidate for future studies.
 
Keywords: HeLa cells, Artemisia kopetdaghensis, Cytotoxicity, Radiotherapy

 

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Current Issue

July-September 2019 (Volume 11, Issue 3)


 

Previous Issue

In the second issue of the journal Electronic Physician for 2019, we have several papers including four Randomized Controlled Trials, a model development study, a case report, an editorial, a letter to editor (LTE), and several original research including two studies with qualitative approach. Authors of this issue are from nine countries: Iran, The Netherlands, Sweden, Italy, India, Thailand, Saudi Arabia, United Arab Emirates (UAE), and Jordan. Read more...


 

The 6th World Conference on Research Integrity (WCRI) is to be held on June 2-5, 2019 in Hong Kong.

The WCRI is the largest and most significant international conference on research integrity. Since the first conference in Lisbon in 2007, it has given researchers, teachers, funding agencies, government officials, journal editors, senior administrators, and research students opportunities to share experiences and to discuss and promote integrity in research. Read more:


 

TDR Clinical Research and Development Fellowships

Call for applications

Deadline for submission: 7 March 2019, 16:00 (GMT)

TDR provides fellowships for early- to mid-career researchers and clinical trial staff (e.g. clinicians, pharmacists, medical statisticians, data managers, other health researchers) in low- and middle-income countries (LMICs) to learn how to conduct clinical trials. Read more:


Meta-Analysis Workshops in New York, USA, and London, UK, in April and May 2019

Don't miss this exceptional opportunity to learn how to perform and report a Meta-analysis correctly. Two Meta-analysis workshops are organized in April and May 2019 by Dr. Michael Borenstein in New York, USA (April 08-10, 2019) and London, UK (May 27-29).

About the Instructor

Dr. Michael Borenstein, one of the authors of Introduction to Meta-Analysis, is widely recognized for his ability to make statistical concepts accessible to researchers as well as to statisticians. He has lectured widely on meta-analysis, including at the NIH, CDC, and FDA. Read more: